Composite

Part:BBa_K3385018:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-13)


CRISPR_spaA_KO


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 489
    Illegal EcoRI site found at 660
    Illegal EcoRI site found at 831
    Illegal SpeI site found at 978
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 489
    Illegal EcoRI site found at 660
    Illegal EcoRI site found at 831
    Illegal NheI site found at 333
    Illegal SpeI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 489
    Illegal EcoRI site found at 660
    Illegal EcoRI site found at 831
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 489
    Illegal EcoRI site found at 660
    Illegal EcoRI site found at 831
    Illegal SpeI site found at 978
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 489
    Illegal EcoRI site found at 660
    Illegal EcoRI site found at 831
    Illegal SpeI site found at 978
    Illegal AgeI site found at 204
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 162


Design Notes

This part was made by digesting the pFC330 backbone with PacI/Nt.BbvCI and assembled by USER cloning It should also be considered to use a non-homologous end joining strain to prevent non-homologous recombination.

Source

This part was obtained through different rounds of PCR amplification and USER cloning.

References